High-Performance Thin-Layer Chromatography (HPTLC) is an advanced form of traditional Thin-Layer Chromatography (TLC) that offers improved separation, higher sensitivity, and reproducibility. It is widely used in pharmaceutical, food, forensic, and environmental analysis for qualitative and quantitative assessments of complex mixtures.
Advantages of HPTLC
- High sample throughput with parallel analysis
- Minimal solvent consumption and eco-friendly
- No extensive sample preparation required
- Multiple detection methods (UV, fluorescence, mass spectrometry)
- Non-destructive and reproducible results
Steps in HPTLC Analysis
- Sample Application – Automated sample spotting ensures uniform and precise application.
- Chromatographic Development – The plate is developed in a controlled chamber under optimized conditions.
- Detection & Visualization – Detection can be done using UV light, visible light, or derivatization agents.
- Documentation – Images and chromatograms are recorded for further analysis.
- Quantification & Interpretation – Densitometry or software-assisted analysis provides quantitative results.
Applications of CAMAG HPTLC System
- Pharmaceutical Analysis – Purity testing, stability studies, herbal medicine standardization.
- Food & Beverage – Detection of contaminants, authenticity verification.
- Forensic Science – Drug and toxin identification.
- Environmental Monitoring – Pollutant detection in water, soil, and air samples.
Learn more about HPTLC
HPTLC instruments for qualitative and quantitative analyses
