Ensuring the authenticity of edible oils is a growing priority in the global food industry. With increasing risks of food fraud and adulteration, advanced analytical techniques are essential to maintain consumer trust and product integrity. High Performance Thin Layer Chromatography (HPTLC) provides a reliable, rapid, and sustainable method for detecting adulteration in oils such as palm, olive, and sunflower. By combining fingerprint profiling with targeted detection, HPTLC enables the identification of both known and unknown contaminants at low concentrations. This innovative approach supports food safety, quality assurance, and the long-term integrity of the food supply chain.